Thursday, May 20, 2010

B Fwd: Re: JCVI publishes booted synthetic genome

---------- Forwarded message ----------
From: "Anselm Levskaya" <>
Date: May 21, 2010 1:19 AM
Subject: Re: JCVI publishes booted synthetic genome
To: <>

I'd like to point out that the -real- news here is the vast array of
techniques that venter's group (led by daniel gibson) has pioneered in
order to build the "assembly stack" to get from noisy 80-mer dna
pieces to a verified megabase.  The techniques are a big deal and
venter is one of the few people adequately funding this kind of purely
technical research.

Two techniques in particular:
- one-step, isothermal assembly of DNA in vitro:
- whole assembly of oligo-tiles into genes in yeast spheroplasts:

are extremely important improvements on past methods both in terms of
efficacy and in terms of their power to construct combinatorial

If any of y'all do cloning in your dayjobs I heavily recommend that
you check out the former method.  I and my colleagues are switching
our entire cloning pipeline to utilize this method.


-- You received this message because you are subscribed to the Google Groups "DIYbio" group. To po...