B Fwd: Re: JCVI publishes booted synthetic genome

---------- Forwarded message ----------
From: "Anselm Levskaya" <levskaya@gmail.com>
Date: May 21, 2010 1:19 AM
Subject: Re: JCVI publishes booted synthetic genome
To: <diybio@googlegroups.com>

I'd like to point out that the -real- news here is the vast array of
techniques that venter's group (led by daniel gibson) has pioneered in
order to build the "assembly stack" to get from noisy 80-mer dna
pieces to a verified megabase.  The techniques are a big deal and
venter is one of the few people adequately funding this kind of purely
technical research.

Two techniques in particular:
- one-step, isothermal assembly of DNA in vitro:
http://www.ncbi.nlm.nih.gov/pubmed/19363495?dopt=Citation
- whole assembly of oligo-tiles into genes in yeast spheroplasts:
http://www.ncbi.nlm.nih.gov/pubmed/19745056?dopt=Citation

are extremely important improvements on past methods both in terms of
efficacy and in terms of their power to construct combinatorial
sequences.

If any of y'all do cloning in your dayjobs I heavily recommend that
you check out the former method.  I and my colleagues are switching
our entire cloning pipeline to utilize this method.

-Anselm

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